Biosintesis dan Karakterisasi Selulosa Bakteri menggunakan Media Sari Pedada (Sonneratia caseolaris) dan Kundur (Benincasa hispida)

Dian Fitriarni(1*), Irianto Sastro Prawiro(2), Nenengsih Verawati(3), Wedi Hardiansyah(4), Dwi Aprianti(5)
(1) Politeknik Negeri Ketapang
(2) Politeknik Negeri Ketapang
(3) Politeknik Negeri Ketapang
(4) Politeknik Negeri Ketapang
(5) Politeknik Negeri Ketapang
(*) Corresponding Author
DOI: http://dx.doi.org/10.25269/jsel.v9i01.250

Abstract

Selulosa bakteri merupakan satu produk yang telah banyak diaplikasikan pada bidang medis, pangan, dan bahkan dapat menjadi bahan baku alternatif untuk mengganti beberapa jenis produk berbahan baku selulosa tumbuhan. Banyak faktor yang berpengaruh terhadap kualitas selulosa bakteri yang dihasilkan seperti bahan baku dan sumber nitrogen untuk media fermentasi. Penelitian ini bertujuan untuk mengetahui karakteristik selulosa bakteri dari hasil fermentasi menggunakan dua media dari sari buah pedada dan kundur dengan berbagai sumber nitrogen berbeda. Sari buah kundur dan sari buah pedada memiliki karakteristik berbeda sehingga mempengaruhi formulasi bahan yang digunakan. Kedua media yang digunakan dimodifkasi dengan penambahan berbagai sumber nitrogen untuk melihat bagaimana karakteristik selulosa bakteri yang dihasilkan. Penelitian dilakukan melalui tahapan eksperimen untuk mendapatkan formulasi yang tepat agar diperoleh ketebalan selulosa maksimal. Berdasarkan hasil karakterisasi selulosa bakteri, berat kering selulosa yang dihasilkan menggunakan media sari buah pedada dengan penambahan urea, yeast, ekstrak kecambah kacang hijau berturut turut 0,37 g/L, 0,52 g/L, dan 2,23 g/L, sedangkan selulosa yang dihasilkan menggunakan media sari buah kundur dengan penambahan urea, yeast, ekstrak kecambah kacang hijau berturut turut 1,3 g/L, 0,32 g/L, dan 5,43 g/L.

 

Abstract

Bacterial cellulose is a product that has been widely applied in the medical, food, and even alternative raw materials for replacing several types of products made from plant cellulose. Many factors influence the quality of bacterial cellulose produced such as raw materials and nitrogen sources for fermentation media. This study aims to determine the characteristics of bacterial cellulose from fermentation using two different media of pedada juice and gourd with various nitrogen sources. Kundur fruit juice and pedada fruit juice have different characteristics so that affect the formulation of the ingredients used. The two media used were modifed by adding various nitrogen sources to see how the characteristics of bacterial cellulose produced. The research was carried out through experimental stages to obtain the right formulation to produce maximum cellulose thickness. Based on the characterization result of bacterial cellulose, the dry weight obtained from the use of pedada fruit juice media using urea, yeast, green bean was in the amount of 0.37 g/L, 0.52 g/L, and 2.23 g/L, respectively. On the other hand bacterial cellulose produced from the use of kundur fruit juice media with the same nitrogen source was obtained with the dry weight of 1.38 g/L, 0.32 g/L, and 5.43 g/L, respectively.

Keywords: bacterial cellulose, pedada, kundur, yeast, green bean



 

Keywords

bacterial cellulose; pedada; kundur; yeast; green bean

Full Text:

PDF

References

Adebayo-Tayo, B., Akintunde, M. and Sanusi, J. (2017) ‘Effect of Different Fruit Juice Media on Bacterial Cellulose Production by Acinetobacter sp. BAN1 and Acetobacter pasteurianus PW1’, Journal of Advances in Biology & Biotechnology, 14(3), pp. 1–9. doi: 0.9734/JABB/2017/34171.

AL-Kalifawi, E. J. and Hassan, I. A. (2014) ‘Factors Influence on the yield of Bacterial Cellulose of Kombucha (Khubdat Humza)’, Baghdad Science Journal, 11(3), pp. 1420–1428. doi: 10.21123/bsj.11.3.1420-1428.

Arviyanti, E. and Yulimartani, N. (2009) Pengaruh Penambahan Air Limbah Tapioka pada Proses Pembuatan Nata. Semarang. Available at: http://eprints.undip.ac.id/3468/.

Barud, H. S. et al. (2008) ‘Self-supported silver nanoparticles containing bacterial cellulose membranes’, Materials Science and Engineering: C, 28(4), pp. 515–518. doi: 10.1016/j.msec.2007.05.001.

Chen, L. et al. (2013) ‘Biotransformation of wheat straw to bacterial cellulose and its mechanism’, Bioresource Technology, 135, pp. 464–468. doi: 10.1016/j.biortech.2012.10.029.

Çoban, E. P. and Biyik, H. (2011) ‘Effect of various carbon and nitrogen sources on cellulose synthesis by acetobacter lovaniensis HBB5’, African Journal of Biotechnology, 10(27), pp. 5346–5354.

Costa, A. F. S. et al. (2017) ‘Production of bacterial cellulose by Gluconacetobacter hansenii using corn steep liquor as nutrient sources’, Frontiers in Microbiology. Frontiers Media S.A., 8(OCT). doi: 10.3389/fmicb.2017.02027.

Djajati, S., Sarofa, U. and Syamsul, A. (2018) ‘Pembuatan nata de manggo (kajian . konsentrasi sukrosa dan lama fermentasi)’, Jurnal Teknologi Pangan, 3(2), pp. 113–127. doi: 10.33005/JTP.V3I2.1022.

Dobre, L. M. et al. (2012) ‘Modelling of sorbic acid diffusion through bacterial cellulose-based antimicrobial films’, Chemical Papers, 66(2), pp. 144–151. doi: 10.2478/s11696-011-0086-2.

Donini, Í. A. N. et al. (2010) ‘Biosynthesis and recent advances in production of bacterial cellulose’, Eclética Química Journal, 35(4), pp. 166–178. doi: 10.26850/1678-4618eqj.v35.4.2010.p165-178.

Esa, F., Tasirin, S. M. and Rahman, N. A. (2014) ‘Overview of Bacterial Cellulose Production and Application’, Agriculture and Agricultural Science Procedia, 2, pp. 113–119. doi: 10.1016/j.aaspro.2014.11.017.

Fifendy, M. and Annisah, N. (2012) ‘Kualitas nata de citrullus dengan menggunakan berbagai macam starter’, Jurnal Sainstek, IV(2), pp. 158–164.

Hastuti, M. et al. (2017) Prosiding SNST. Semarang: Fakultas Teknik, Universitas Wahid Hasyim.

Horwitz, W. and Latimer, G. (eds) (2010) Official Methods of Analysis of AOAC International. 18th edn.

Indrianingsih, A. W. et al. (2017) ‘Preliminary study on biosynthesis and characterization of bacteria cellulose films from coconut water’, in IOP Conference Series: Earth and Environmental Science, pp. 1–8. doi: 10.1088/1755-1315/101/1/012010.

Kosseva, M. R. et al. (2017) ‘Study on The Bacterial Cellulose Production From Fruit Juices’, Bioscience and Biotechnology, 2, pp. 36–42. doi: 10.17501/biotech.2017.2104.

Majesty, J. et al. (2015) Pengaruh Penambahan Sukrosa dan Lama Fermentasi Terhadap Kadar Serat Nata Dari Sari Nanas (Nata de Pina), Jurnal Keteknikan Pertanian Tropis dan Biosistem.

Molina-Ramírez, C. et al. (2017) ‘Effect of different carbon sources on bacterial nanocellulose production and structure using the low pH resistant strain Komagataeibacter medellinensis’, Materials. MDPI AG, 10(6 (639)), pp. 1–13. doi: 10.3390/ma10060639.

Moniri, M. et al. (2017) ‘Production and status of bacterial cellulose in biomedical engineering’, Nanomaterials, 7(257), pp. 1–26. doi: 10.3390/nano7090257.

Mumbarkar, V. and Shravya, D. (2013) ‘Evaluation of Protein Content among Sprouted and Un-Sprouted Seeds of Selected Pulses’, Helix, 4, pp. 374–377.

Nazeri, M. A. (2012) Optimization of bacterial cellulose production by using response surface methodology (RSM): the effect of pH, temperature and concentration of fermentation medium.

Neera, Ramana, K. V. and Batra, H. V. (2015) ‘Occurrence of Cellulose-Producing Gluconacetobacter spp. in Fruit Samples and Kombucha Tea, and Production of the Biopolymer’, Applied Biochemistry and Biotechnology. Humana Press Inc., 176(4), pp. 1162–1173. doi: 10.1007/s12010-015-1637-8.

Rebelo, A. et al. (2018) ‘Dehydration of bacterial cellulose and the water content effects on its viscoelastic and electrochemical properties’, Science and Technology of Advanced Materials, 19(1), pp. 203–211. doi: 10.1080/14686996.2018.1430981.

Surma-Ślusarska, B., Presler, S. and Danielewicz, D. (2008) ‘Characteristics of Bacterial Cellulose Obtained from Acetobacter Xylinum Culture for Application in Papermaking’, Fibres & Textile in Eastern Europe, 16(4 (69)), pp. 108–111.

Tsouko, E. et al. (2015) ‘Bacterial cellulose production from industrial waste and by-product streams’, International Journal of Molecular Sciences, 16(7), pp. 14832–14849. doi: 10.3390/ijms160714832.

Wijayanti, F., Kumalaningsih, S. and Effendi, M. (2012) ‘Pengaruh penambahan sukrosa dan asam asetat glacial terhadap kualitas nata dari whey tahu dan substrat air kelapa’, Jurnal Industria, 1(2), pp. 86–93.

Yang, J. et al. (2009) ‘In situ deposition of platinum nanoparticles on bacterial cellulose membranes and evaluation of PEM fuel cell performance’, Electrochimica Acta, 54(26), pp. 6300–6305. doi: 10.1016/J.ELECTACTA.2009.05.073.

Yang, Z. et al. (2012) ‘Flexible luminescent CdSe/bacterial cellulose nanocomoposite membranes’, Carbohydrate Polymers, 88(1), pp. 173–178. doi: 10.1016/J.CARBPOL.2011.11.080.

Yustinah, Y. (2012) ‘Pengaruh jumlah sukrosa pada pembuatan nata de pina dari sari buah nanas’, Jurnal Konversi, 1(1), pp. 29–36. doi: 10.24853/KONVERSI.1.1.%P.

Zahan, K. ., Hedzir, M. S. . and Mustapha, M. (2017) ‘The Potential Use of Papaya Juice as Fermentation Medium for Bacterial Cellulose Production by Acetobacter xylinum 0416’, Pertanika J. Trop. Agric. Sci, 40(3), pp. 343–350.


Article Metrics


Abstract view : 4 times
PDF view : 1 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 JURNAL SELULOSA
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.