Pengaruh Sumber Karbon pada Produksi Lakase dari Jamur Pelapuk Putih Marasmius sp. dalam Fermentasi Kultur Padat

Hendro Risdianto(1*), Elis Sofianti(2), Suraya Suraya(3), Sri Harjati Suhardi(4), Tjandra Setiadi(5)
(1) Center for Pulp and Paper, Ministry of Industry
(2) Department of Chemical Engineering, Institut Teknologi Bandung
(3) School of Life Sciences and Engineering, Institut Teknologi Bandung
(4) School of Life Sciences and Engineering, Institut Teknologi Bandung
(5) Department of Chemical Engineering, Institut Teknologi Bandung
(*) Corresponding Author


Lakase merupakan salah satu enzim ligninolitik yang memiliki kemampuan mendegradasi lignin. Lakase telah diproduksi menggunakan jamur pelapuk putih Marasmius sp. dalam Fermentasi Kultur Padat (FKP) menggunakan jerami padi sebagai media pertumbuhan. Pengaruh sumber karbon yaitu glukosa, gliserol, dan molase dalam medium produksi lakase digunakan dalam penelitian ini. Konsentrasi 0,5%; 1,0%; dan 2,0% digunakan untuk tiap jenis sumber karbon. Hasil menunjukkan bahwa aktivitas tertinggi lakase diperoleh pada kultivasi hari ke 6-10  dengan masing-masing aktivitas (872,0 U/L (hari ke-6), 1516,67 U/L (hari ke-9) dan 1270,69 U/L (hari ke-10). Aktivitas lakase tertinggi diperoleh pada penggunaan medium gliserol dan molase masing-masing adalah 1422,36 U/L (pada konsentrasi 1%, hari ke-7) dan 113,19 U/L (pada konsentrasi 2%, hari ke-8). Aktivitas tertinggi tersebut sebanding dengan penggunaan medium glukosa. Oleh karena itu, gliserol dan molase dapat digunakan sebagai alternatif sumber karbon untuk produksi lakase dengan fermentasi kultur padat.

Kata kunci: glukosa, gliserol, lakase, molase, Marasmius sp., fermentasi kultur padat


Influence of Carbon Sources on Laccase Production by White Rot Fungus Marasmius sp. in Solid State Fermentation


Laccase is an one of the ligninolytic enzymes that capable to degrade lignin in biomass. Laccase has been produced by white rot fungus Marasmius sp. in Solid State Fermentation (SSF) using rice straw as the solid support media. The influence of carbon sources, i.e. glucose, glycerol and molasses in medium of laccase production were studied in this paper. The concentration of 0.5%, 1.0% and 2.0% were used for each carbon sources. The results showed that the highest lacase activity was obtained within 6-10 days of cultivation. Glucose concentration of 0.5%, 1.0% and 2.0% gave the highest laccase activity were 872.0 U/L (day 6), 1516.67 U/L (day 9) and 1270.69 U/L (day 10) respectively. The highest laccase activity on using glycerol and molasses was 1422.36 U/L (at concentration of 1 % on day 7th) and 1113.19 U/L (at concentration of 2% on day 8th), respectively. This activity was comparable to that of glucose substrate. Therefore, glycerol and molasses gave a potential chance as carbon sources for the strategy on low cost laccase production in solid state fermentation.

Keywords: glucose, glycerol, laccase, molasses, Marasmius sp., solid state fermentation. 


glucose, glycerol, laccase, molasses; Marasmius sp.; solid state fermentation

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Asadgol, Z. et al. (2014) ‘Removal of phenol and bisphenol-a catalyzed by laccase in aqueous solution’, Journal of Environmental Health Science and Engineering, 12(1). doi: 10.1186/2052-336X-12-93.

Bertrand, B. et al. (2015) ‘Biochemical and molecular characterization of laccase isoforms produced by the white-rot fungus Trametes versicolor under submerged culture conditions’, Journal of Molecular Catalysis B: Enzymatic, 122, pp. 339–347. doi: 10.1016/j.molcatb.2015.10.009.

Elisashvili, V., Kachlishvili, E. and Penninckx, M. (2008) ‘Effect of growth substrate, method of fermentation, and nitrogen source on lignocellulose-degrading enzymes production by white-rot basidiomycetes’, in Journal of Industrial Microbiology and Biotechnology, pp. 1531–1538. doi: 10.1007/s10295-008-0454-2.

Feijoo, G., Dosoretz, C. and Lema, J. M. (1995) ‘Production of lignin peroxidase by Phanerochaete chrysosporium in a packed bed bioreactor operated in semi-continuous mode’, Journal of Biotechnology, 42(3), pp. 247–253. doi: 10.1016/0168-1656(95)00085-5.

Forootanfar, H. et al. (2013) ‘Synthetic dye decolorization by three sources of fungal laccase’, Research Journal of Chemistry and Environment, 17(5), pp. 76–81. doi: 10.1186/1735-2746-9-27.

Li, P. et al. (2011) ‘The effect of carbon source succession on laccase activity in the co-culture process of Ganoderma lucidum and a yeast’, Enzyme and Microbial Technology, 48(1), pp. 1–6. doi: 10.1016/j.enzmictec.2010.07.005.

Marim, R. A. et al. (2016) ‘Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization’, Genetics and Molecular Research, 15(4), pp. 1–9. doi: 10.4238/gmr15048972.

Martins, L. O. et al. (2015) ‘Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology’, Cellular and Molecular Life Sciences, 72(5), pp. 911–922. doi: 10.1007/s00018-014-1822-x.

Mazumder, S., Basu, S. K. and Mukherjee, M. (2009) ‘Laccase production in solid-state and submerged fermentation by Pleurotus ostreatus’, Engineering in Life Sciences, 9(1), pp. 45–52. doi: 10.1002/elsc.200700039.

Montoya, S., Sánchez, Ó. J. and Levin, L. (2014) Mathematical modeling of lignocellulolytic enzyme production from three species of white rot fungi by solid-state fermentation, Advances in Intelligent Systems and Computing. doi: 10.1007/978-3-319-01568-2_52.

Muthukumar, N. P. and Murugan, S. (2014) ‘Production, Purification and Application of Bacterial Laccase: A Review’, Biotechnology(Faisalabad), 13(5), pp. 196–205. doi: 10.3923/biotech.2014.196.205.

Nadeem, A., Baig, S. and Sheikh, N. (2014) ‘Mycotechnological production of laccase by Pleurotus ostreatus-P1 and its inhibition study’, J. Anim. Plant Sci, 24(2), pp. 492–502.

Niku-Paavola, M. L. et al. (1988) ‘Ligninolytic enzymes of the white-rot fungus Phlebia radiata.’, The Biochemical journal, 254(3), pp. 877–883. doi: 10.1042/bj2540877.

Osma, J. F., Toca Herrera, J. L. and Rodríguez Couto, S. (2007) ‘Banana skin: A novel waste for laccase production by Trametes pubescens under solid-state conditions. Application to synthetic dye decolouration’, Dyes and Pigments, 75(1), pp. 32–37. doi: 10.1016/j.dyepig.2006.05.021.

Pandey, A. (2003) ‘Solid-state fermentation’, Biochemical Engineering Journal, 13(2–3), pp. 81–84. doi: 10.1016/S1369-703X(02)00121-3.

Rencoret, J. et al. (2017) ‘Delignification and Saccharification Enhancement of Sugarcane Byproducts by a Laccase-Based Pretreatment’, ACS Sustainable Chemistry and Engineering, 5(8), pp. 7145–7154. doi: 10.1021/acssuschemeng.7b01332.

Rezaei, S. et al. (2015) ‘Efficient decolorization and detoxification of reactive orange 7 using laccase isolated from Paraconiothyrium variabile, kinetics and energetics’, Journal of the Taiwan Institute of Chemical Engineers, 56, pp. 113–121. doi: 10.1016/j.jtice.2015.04.008.

Risdianto, H. et al. (2008) ‘Produksi lakase dan potensi aplikasinya dalam proses pemutihan pulp’, Jurnal Selulosa, 43(01), pp. 1–10.

Risdianto, H. et al. (2012) ‘Optimisation of laccase production using white rot fungi and agriculture wastes in solid state fermentation’, ITB Journal of Engineering Science, 44 B(2). doi: 10.5614/itbj.eng.sci.2012.44.2.1.

Sánchez, C. (2009) ‘Lignocellulosic residues: Biodegradation and bioconversion by fungi’, Biotechnology Advances, pp. 185–194. doi: 10.1016/j.biotechadv.2008.11.001.

Sharma, A. et al. (2014) ‘Xylanase and laccase based enzymatic kraft pulp bleaching reduces adsorbable organic halogen (AOX) in bleach effluents: A pilot scale study’, Bioresource Technology, 169, pp. 96–102. doi: 10.1016/j.biortech.2014.06.066.

da Silva, G. P., Mack, M. and Contiero, J. (2009) ‘Glycerol: A promising and abundant carbon source for industrial microbiology’, Biotechnology Advances, pp. 30–39. doi: 10.1016/j.biotechadv.2008.07.006.

Soccol, C. R. et al. (2017) ‘Recent developments and innovations in solid state fermentation’, Biotechnology Research and Innovation, pp. 52–71. doi: 10.1016/j.biori.2017.01.002.

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